IgG4 Detection of Echinococcus granulosus Paramyosin Is a Useful Diagnostic Test for Human Hydatidosis
نویسندگان
چکیده
منابع مشابه
Serological Evaluation of EgAgB16 kDa, a Recombinant Antigen from Echinococcus granulosus for Diagnosis of Human Hydatidosis
BACKGROUND Regarding that accurate diagnosis of human hydatidosis still needs more investigations, the present study was conducted to clone, express, and evaluate the gene encoding AgB subunits (EgAgB16 kDa) from Echinococcus granulosus (Iranian G1 strain) and its evaluation by ELISA test. METHODS DNA was extracted from protoscoleces and was utilized by PCR for strain identification. Total RN...
متن کاملDetection of Echinococcus granulosus infection in dogs
Cystic Echinococcosis is an important zoonosis in the sheep rising areas of Iran. To develop a simple and reliable diagnostic method for Echinococcus infection in definitive hosts, E. granulosus polyoclonal antibodies (PolyAbs) were prepared from adult worm in rabbit. A selected PoAb was used for coproantigen detection in faecal samples obtained from animals naturally infected with Echinococcus...
متن کاملserological evaluation of egagb16 kda, a recombinant antigen from echinococcus granulosus for diagnosis of human hydatidosis
background: regarding that accurate diagnosis of human hydatidosis still needs more investigations, the present study was conducted to clone, express, and evaluate the gene encoding agb subunits (egagb16 kda) from echinococcus granulosus (iranian g1 strain) and its evaluation by elisa test. methods: dna was extracted from protoscoleces and was utilized by pcr for strain identification. total rn...
متن کاملIdentification of Six Introns in a Partial Sequence of Echinococcus granulosus Paramyosin Gene.
OBJECTIVE Paramyosin is a major protein produced by the metacestode cyst of Echinococcus granulosus, the causative agent of cystic hydatid disease. This protein has been shown to play an important role in modulating host immune responses. In this study, we attempted to characterize the noncoding sequence of the paramyosin gene. METHODS Genomic DNA was isolated from G1 Iranian hydatid cysts. A...
متن کاملClinical evaluation of a complement fixation test using human hydatid cyst fluid antigen for the detection of Echinococcus granulosus antibody
Complement fixation (CF) is a commonly used serodiagnostic test for hydatid disease, especially recommended for the evaluation of cure after surgery or for checking on treatment with antihelminthic drugs (9). However, the absence of an FDA-approved standardized antigen makes it difficult to assess the performance of different CF tests by using published studies. The objectives of this study wer...
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ژورنال
عنوان ژورنال: Clinical and Vaccine Immunology
سال: 2013
ISSN: 1556-6811,1556-679X
DOI: 10.1128/cvi.00019-13